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- Order number: 7TM0113N-IC
- Content: 100 µl
- Host: Rabbit
The GPR65 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GRP65. It can be used to detect total GPR65 receptors in Western blots independent of phosphorylation. The GPR65 antibody can also be used to isolate and enrich GPR65 receptors from brain lysates. It also detects GPR65 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | Dig1 | Gpcr25 | Psychosine receptor | TDAG8 | T cell death associated protein 8 |
| IUPHAR Target ID | 113 |
| UniProt ID | Q8IYL9 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide presents distal end of carboxy-terminal tail of human GPR65. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Immunohistochemical identification of G Protein-coupled Receptor 65 in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR65 (G Protein-coupled Receptor 65) antibody (7TM0113N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Figure 2. Validation of the GPR65 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR65 Receptor (GPR65) were lysed and immunoblotted with the phosphorylation-independent anti-GPR65 antibody (7TM0113N-IC) at a dilution of 1:1000.
Figure 3. Immunohistochemical varification of GPR65 Receptor antibody in enteric ganglion. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GPR65 (non-phospho-GPR65 Receptor)) antibody (7TM0113N-IC). Sections were then incubated with anti-GPR65 (non-phospho-GPR65 Receptor) antibody (7TM0113N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GPR65 receptors were uniformly detected at the plasma membrane of cells.
Figure 4. Immunohistochemical identification of G Protein-coupled Receptor 65 in human cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR65 (G Protein-coupled Receptor 65) antibody (7TM0113N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Figure 5. Immunohistochemical identification of G Protein-coupled Receptor 65 in rat nucleus accumbens. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR65 (G Protein-coupled Receptor 65) antibody (7TM0113N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
