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- Order number: 7TM0080N-GP
- Content: 100 µl
- Host: Rabbit
The non-phospho-ACKR3 receptor antibody is directed against the carboxyl-terminal tail of mouse, rat and human ACKR3. It can be used to detect total ACKR3 receptors in Western blots independent of phosphorylation. The non-phospho-ACKR3 antibody can also be used to isolate and enrich ACKR3 receptors from cell and tissue lysates. The ACKR3 antibody can also be used to detect ACKR3 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
Alternative Names | ACKR3, CXCR7, Atypical Chemokin Receptor 3, CXC Chemokin Receptor 7 |
IUPHAR Target ID | 80 |
UniProt ID | P25106 |
Immunohistochemistry (IHC) | 1:100 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Guinea Pig / IgG |
Class | Polyclonal |
Immunogen | A synthetic peptide presents distal end of carboxyl-terminal tail of human ACKR3. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the Atypical Chemokine Receptor 3 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 3 (ACKR3) were lysed and immunoblotted with the phosphorylation-independent c-terminal anti-ACKR3 antibody (7TM0080N-GP) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of Atypical Chemokine Receptor 3 in Heart. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ACKR3 (Atypical Chemokine Receptor 3) antibody (7TM0080N-GP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.