CCR10 (non-phospho) Chemokine Receptor 10 Antibody

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Preview: Validation of the CC Chemokine Receptor 10 in transfected HEK293 cells

Preview: Immunohistochemical identification of CC Chemokine Receptor 10 in liver

Preview: Immunohistochemical identification of CC Chemokine Receptor 10 in kidney

Validation of the CC Chemokine Receptor 10 in transfected HEK293 cells

Immunohistochemical identification of CC Chemokine Receptor 10 in liver

Immunohistochemical identification of CC Chemokine Receptor 10 in kidney

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Order number: 7TM0067N
Content: 100 µl
Host: Rabbit

The non-phospho-CCR10 receptor antibody is directed against the distal end of the... more

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The non-phospho-CCR10 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human CCR10. It can be used to detect total CCR10 receptors in Western blots independent of phosphorylation. The CCR10 antibody can also be used to isolate and enrich CCR10 receptors from tissue lysates. It also detects CCR10 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names CCR10, GPR-2, Chemokine Receptor 10 IUPHAR Target... more

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Alternative Names	CCR10, GPR-2, Chemokine Receptor 10
IUPHAR Target ID	67
UniProt ID	P46092
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to c-terminal end of human CCR10.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the CC Chemokine Receptor 10 in transfected HEK293 cells. Native... more

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Figure 1. Validation of the CC Chemokine Receptor 10 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the CC Chemokine Receptor 10 (CCR10) were lysed and immunoblotted with the anti-CCR10 antibody (7TM0067N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of CC Chemokine Receptor 10 in liver. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CCR10 (CC Chemokine Receptor 10) antibody (7TM0067N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of CC Chemokine Receptor 10 in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CCR10 (CC Chemokine Receptor 10) antibody (7TM0067N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.