pS326/pT327/pS328-HCA2 (phospho-Hydroxycarboxylic Acid Receptor 2 Antibody)

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Agonist-induced Serine326/Threonine327/Serine328 phosphorylation of the Hydroxycarboxylic Acid Receptor 2

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Order number: 7TM0312B
Content: 100 µl
Host: Rabbit

Serine326/Threonine327/Serine328 (S326/T327/S328) is major phosphorylation site of... more

Serine326/Threonine327/Serine328 (S326/T327/S328) is major phosphorylation site of the Hydroxycarboxylic Acid Receptor 2 (HCA2). The pS326/pT327/pS328-HCA2 antibody detects phosphorylation in response to agonists. S326/T327/S328 phosphorylation is likely to be involved in efficient ligand sequestration.

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Alternative Names PUMAG, Nicotinic acid receptor, G-protein coupled... more

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Alternative Names	PUMAG, Nicotinic acid receptor, G-protein coupled receptor 109A, Niacin receptor 1
IUPHAR Target ID	312
UniProt ID	Q8TDS4
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human HCA2 around the phosphorylation site of Ser326/Thr327/Ser328.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine326/Threonine327/Serine328 phosphorylation of the... more

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Figure 1. Agonist-induced Serine326/Threonine327/Serine328 phosphorylation of the Hydroxycarboxylic Acid Receptor 2. Upper panel, HEK293 cells stably expressing the Hydroxycarboxylic acid receptor 2 (HCA2) and transiently GRK2 (Ratio HCA2 receptor:GRK2 = 10:1) were preincubated with serum-free media. After 2 hours cells were either not exposed or exposed to 100 nM Nicotinic Acid for 30 minutes. Cells were lysed and immunoblotted with the anti-pS326/pT327/pS328-HCA2 antibody (7TM0312B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-HCA2 antibody to confirm equal loading of the gel.

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