MCH1 (non-phospho) Melanin-Concentrating Hormone Receptor 1 Antibody

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Preview: Validation of the Melanin-Concentrating Hormone Receptor 1 in transfected HEK293 cells

Preview: Immunohistochemical identification of Melanin-Concentrating Hormone Receptor 1 in pancreas

Validation of the Melanin-Concentrating Hormone Receptor 1 in transfected HEK293 cells

Immunohistochemical identification of Melanin-Concentrating Hormone Receptor 1 in pancreas

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Order number: 7TM0280N
Content: 100 µl
Host: Rabbit

The non-phospho MCH1 receptor antibody is directed against the distal end of the... more

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The non-phospho MCH1 receptor antibody is directed against the distal end of the carboxyl-terminal tail human MCH1 receptor. It can be used to detect total MCH1 receptors in Western blots independent of phosphorylation. It can also be used to isolate and enrich MCH1 receptors from tissue lysates. It also detects MCH1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names GPR24 | MCH1R | G protein-coupled... more

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Alternative Names	GPR24 \| MCH1R \| G protein-coupled receptor 24 \| MCH receptor 1 \| somatostatin receptor-like protein
IUPHAR Target ID	280
UniProt ID	Q99705
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human MCH1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Melanin-Concentrating... more

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Figure 1. Validation of the Melanin-Concentrating Hormone Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Melanin-Concentrating Hormone Receptor 1 (MCH1) were lysed and immunoblotted with the anti-MCH1 antibody (7TM0280N) at a dilution of 1:1000.

Figure 1. Immunohistochemical identification of Melanin-Concentrating Hormone Receptor 1 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-MCH1 (Melanin-Concentrating Hormone Receptor 1) antibody (7TM0280N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

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