OX2 (IHC-grade) Orexin Receptor 2 Antibody

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Preview: Immunohistochemical identification of Orexin Receptor 2 in pancreas

Preview: Validation of the Orexin Receptor 2 in transfected HEK293 cells

Immunohistochemical identification of Orexin Receptor 2 in pancreas

Validation of the Orexin Receptor 2 in transfected HEK293 cells

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Order number: 7TM0322N-IC
Content: 100 µl
Host: Rabbit

The non-phospho OX2 receptor antibody is directed against the distal end of the... more

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The non-phospho OX2 receptor antibody is directed against the distal end of the carboxyl-terminal tail human OX2 receptor. It can be used to detect total OX2 receptors in Western blots independent of phosphorylation. It can also be used to isolate and enrich OX2 receptors from tissue lysates. It also detects OX2 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names OX2R, Hypocretin Receptor 2 IUPHAR Target ID... more

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Alternative Names	OX2R, Hypocretin Receptor 2
IUPHAR Target ID	322
UniProt ID	O43614
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human OX2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Orexin Receptor 2 in pancreas. Sections... more

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Figure 1. Immunohistochemical identification of Orexin Receptor 2 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-OX2 (non-phospho-Orexin Receptor 2) antibody (7TM0322N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Validation of the Orexin Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Orexin Receptor 2 (OX2) were lysed and immunoblotted with the anti-OX2 antibody (7TM0322N-IC) at a dilution of 1:1000.

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