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- Order number: 7TM0166N-GP
- Content: 100 µl
- Host: Guinea Pig
The non-phospho-SUCNR1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human SUCNR1. It can be used to detect total SUCNR1 receptors in Western blots independent of phosphorylation. The SUCNR1 antibody can also be used to isolate and enrich SUCNR1 receptors from tissue lysates.
| Alternative Names | succinate receptor 1, GPR91, P2Y purinoceptor 1 |
| IUPHAR Target ID | 166 |
| Uniprot ID | Q9BXA5 |
| Western Blot (WB) | 1:1000 |
| Species Reactivity | Human |
| Host / Isotype | Guinea Pig / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to distal end of carboxy-terminal tail of human SUCNR1/GPR91. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Indentification of SUCNR1 Receptor in mouse tissues. Native mouse tissues were lysed and GPCR receptors were enriched by using WGA-beads (see 7TM Western Blot Protocol). Extracted proteins were immunoblotted with the phosphorylation-independent anti-SUCNR1 antibody (7TM0166N-GP) at a dilution of 1:1000.
Figure 2. Validation of the SUCNR1 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the SUCNR1 Receptor were lysed and immunoblotted with the phosphorylation-independent anti-SUCNR1 antibody (7TM0166N-GP) at a dilution of 1:1000